Rockefeller University Research Assistant in New York, New York
Job Title Research Assistant
Laboratory / Department Molecular and Cellular Neuroscience
The Laboratory of Molecular and Cellular Neuroscience studies the molecular defects responsible for neurological and psychiatric disorders including Alzheimer's disease, major depressive disorder, Parkinson's disease, and obsessive compulsive disorder, as well as the molecular mechanisms by which neuro- and psychoactive drugs produce their pharmacological actions in these disorders. Research from the laboratory has demonstrated that most neurotransmitters and neuromodulators achieve their actions and interactions on post-synaptic neurons through a process called "slow synaptic transmission." This process involves activation of highly complex signal transduction cascades. For the last 10 years, the group has applied this knowledge and developed a state-of-the-art methodologies (eg. BacTRAP), to the study of the molecular pathways behind various neurological and psychiatric disorders.
The Research Assistant will perform experiments aimed at investigating selective neuronal vulnerability in Alzheimer's disease using human stem cells. Experiments will include differentiation of stem cells and direct differentiation of human fibroblast towards neuronal and glia identity in vitro, CRISPR-cas9 gene editing in stem cells, cell culture, immunocytochemistry, plasmid purification, PCR and ELISA. The Research Assistant will be responsible for routine lab maintenance, set-ups and operation of various scientific apparatus, and compiling, processing and analyzing data.
Bachelor's degree in science required; educational emphasis or experience in molecular/cellular biology preferred. Neuroscience background is a plus. Must have knowledge of laboratory procedures and proficiency in at least some of the following techniques: cell culture, antibody staining, molecular biology, PCR, and Western blot. Must be meticulous, analytic and extremely motivated.