Rockefeller University Research Specialist in New York, New York
Job Title Research Specialist
Laboratory / Department 114120 Ruta Laboratory
The Laboratory of Neurophysiology and Behavior takes advantage of the relatively simple nervous system of the fruit fly Drosophila to examine how flexibility is engineered into the nervous system to give rise to adaptive behaviors, at the level of both individuals and species. We examine how learning rapidly shapes neural pathways to allow each member of a species to respond appropriately to their unique experience of the world. We also consider how neural circuits are adapted over longer evolutionary time scales to give rise to species-specific behaviors. Our lab employs a multidisciplinary approach, from biochemistry to behavior, with the goal of revealing the neural basis for flexible behavior at high resolution.
The Laboratory of Neurophysiology and Behavior is seeking an experienced and talented molecular biologist who will contribute to ongoing work studying how neural circuits evolve across Drosophila species to mediate species-specific behaviors. The work will include designing and implementing different genome editing strategies, such as PiggyBac, PhiC31, and CRISPR-Cas9, to generate novel genetic reagents in non-model Drosophila species. Responsibilities include designing and constructing targeting vectors, screening transformants by fluorescent markers and/or genotyping, and Drosophila husbandry. Additional duties may consist of anatomic, functional, or behavioral characterization of generated lines. Will be an active contributor to research projects, including in the conceptual design and analysis of experiments, and presentation of data in laboratory meetings.
Bachelor's degree in science required; Master's degree and educational emphasis in molecular biology, genetics, or a closely related field preferred. Extensive experience in molecular biology, including CRISPR-Cas9 genome editing and experience designing complex cloning strategies and troubleshooting molecular biology experiments required. Must have excellent organizational, communication, and time management skills. Must be detail-oriented, highly organized, focused, a fast learner, and able to work within a team as well independently. Experience working with Drosophila and a solid foundation in fly genetics, desirable. Expertise in a subset of the following techniques is highly desirable: DNA/RNA extraction, PCR (conventional, multiplex, touchdown, reverse transcription, genotyping), cloning (restriction enzyme, Gibson assembly, Gateway), CRISPR-Cas9 genome editing, Drosophila genetics, or Drosophila husbandry. Must be detail-oriented, focused, and able to work within a team as well as independently. The ability to be creative and stay abreast of advanced techniques in molecular biology and be proactive and take ownership of your work necessary. Must be excited to contribute to innovative science, work independently and manage multiple projects concurrently.
This job requires that employees be fully vaccinated against COVID-19 as a condition of employment. Rockefeller University provides reasonable accommodations where legally required including accommodations for medical conditions and sincerely held religious beliefs.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans