Organization Overview

The Proteomics Resource Center at The Rockefeller University masters most aspects of mass spectrometry based proteomics which includes denovo sequencing, targeted studies, quantitative proteomics profiling based on label-free quantitation as well as metabolic labelled samples (SILAC), chemical labeling, tandem-mass tag technology, absolute quantitation, and global post-translational analysis. The Center also offers LC-MS based analysis of small molecules, polar metabolites, and lipids. Very importantly, the Center is a source for help with planning of mass spectrometry-based experiments, and the Center have the capability to offer in-depth collaborative analysis. Also, the PRC is operated in a boutique style, encouraging the scientists of the Center to work closely with users to create a tailored approach to fit the many unique questions that can be answered by analytical mass spectrometry. The PRC is equipped with Orbitrap-type mass spectrometers (high resolution/high mass accuracy), operates a triple quadrupole, and can separate analytes using both nano and high-flow liquid chromatography. The Center is equipped with multiple high-performance servers for data analysis. In addition to analysis by mass spectrometry, the Center offers production of custom peptides and peptide libraries and different pre-mass spectrometry fractionation techniques, which include off-line separation of peptides and proteins by liquid chromatography.

Overview

Reporting to the Metabolomics Platform Manager, the Research Support Assistant will focus on the Metabolomics Platform at the Proteomics Resource Center (PRC).

Responsibilities

Responsibilities include:

• Sample preparation

• Maintain and operate LC-MS/MS equipment

• Analyze metabolomic and lipidomic projects in close collaboration with colleagues and researchers from a wide variety of university laboratories

• Consult with Metabolomics Platform Manager to identify project goals, experimental design and deadlines

• Interact with team members in support of overall Center operations

• Comply with lab safety guidelines

• Track samples and submit billing and purchasing information

• Maintain lab records

• Generate detailed technical documents and sample operating procedures

• Maintain laboratory appearance and monitor chemical inventory and supplies

• Manage and expand compound libraries

• Maintain and operate LC-MS (Q-Exactive Plus and Orbitrap IQ-X) and related equipment with a focus on high quality data generation

• Prepare samples from a variety of biological matrices, using various sample processing techniques.

Qualifications

• Bachelor's degree in chemistry or related field

• Excellent communication (oral and written English) and interpersonal skills

• Strong organizational and multitasking skills

• The ability to work independently as well as part of a team

• The ability to keep accurate and detailed records in a lab notebook and LIMS system

• Strong analytical and wet lab skills including but not limited to:

• Stoichiometry calculations

• Use of an analytical balance/ pH meter/centrifuge

• Pipetting (single and multi-channel)

• Solution/buffer preparation

• Serial dilutions

Experience and understanding of liquid chromatography and or mass spectrometry preferred.

The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans.

Compensation Range: Min

USD $50,000.00/Yr.

Compensation Range: Max

USD $60,000.00/Yr.

Job Locations US-NY-New York

Category Science

Position Type Regular Full-Time