Rockefeller University Research Support Specialist in New York, New York
Job Title Research Support Specialist
Laboratory / Department Cryo-Electron Microscopy Resource Center
The Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center (CEMRC) provides its users with a world class environment to make advances in structural biology. The center is equipped with three high end dedicated cryo-electron microscopes. The microscopy suite was meticulously and specially designed to allow these instruments to perform beyond their specified resolutions. This center provides users with the world's most stable dedicated cryo-electron microscopes optimized for high resolution single particle analysis of proteins and protein complexes as well as high resolution cellular tomography. Users work alongside CEMRC staff until they have mastered electron microscope operation at which point they work autonomously.
The Research Support Specialist will provide training and support to users of the center in sample preparation, microscope operation, high-throughput data acquisition, and data analysis. The position entails a specialized focus in establishing cryo-electron tomography (cryo-ET) workflows for recently acquired instrumentation for the Center, including an Aquilos cryo-Focused Ion Beam (cryo-FIB) milling system and a Titan Krios featuring Cs correction, Volta phase plates, and a Gatan BioQuantum energy filter / K3 direct electron detector. The specialist will also assist in the technical and administrative functions of the center, including scheduling, tracking use, and supply management, establish protocols and train users in cryo-FIB milling utilizing the center's Aquilos cryo-FIB instrument and in cryo-ET tilt series data acquisition utilizing the center's Talos Arctica and 2 Titan Krios systems, assist users with image processing for tomographic reconstruction and subtomogram averaging, and support the center's ongoing efforts in single-particle cryo-EM data acquisition and image processing.
Bachelor's degree in science required; educational emphasis in biological or physical sciences, engineering, mathematics, computer science, or related fields preferred. Advanced degree in these fields preferred. A minimum of three years of training related to cry-electron microscopy image processing, which includes prior experience with cryo-electron tomography data acquisition and analysis. Previous experience in cryo-FIB milling and/or correlative light and electron microscope and in single particle cryo-EM data acquisition and analysis a plus. Excellent communication, organizational and interpersonal skills necessary.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans